hybe buffer (Thermo Fisher)

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Thermo Fisher hybe buffer
Hybe Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+(sspe)/SSPE/pmc12802891-259-6-12
Average 94 stars, based on 1 article reviews

hybe buffer - by Bioz Stars, 2026-11

94/100 stars

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Polymerase Chain Reaction:

Article Title: Pathogen diversity of the neglected winter tick Alloceraea inermis in Hungary
Article Snippet: .. The resulting PCR products from each tick were pooled individually in 2× SSPE buffer (Thermo Scientific, Vienna, Austria), which contained 0.3M NaCl, 0.02M NaH 2 PO 4 , 0.002M EDTA, and 0.1% sodium dodecyl sulfate (SDS, Applichem, Darmstadt, Germany). .. The resulting PCR products from each tick were pooled individually in 2× SSPE buffer (Thermo Scientific, Vienna, Austria), which contained 0.3M NaCl, 0.02M NaH 2 PO 4 , 0.002M EDTA, and 0.1% sodium dodecyl sulfate (SDS, Applichem, Darmstadt, Germany).

Hybridization:

Article Title: SMYD5 methylation of rpL40 links ribosomal output to gastric cancer.
Article Snippet: Dysregulated transcription due to disruption in histone lysine methylation dynamics is an established contributor to tumorigenesis.. However, whether analogous pathologic epigenetic mechanisms act directly on the ribosome to advance oncogenesis is unclear.. Here we find that trimethylation of the core ribosomal protein L40 (rpL40) at lysine 22 (rpL40K22me3) by the lysine methyltransferase SMYD5 regulates mRNA translation output to promote malignant progression of gastric adenocarcinoma (GAC) with lethal peritoneal ascites.

Article Title: Innate immune training restores pro-reparative myeloid functions to promote remyelination in the aged central nervous system.
Article Snippet: .. Oligo dT25 magnetic beads (61005, Invitrogen) were prepared with three washes of 1x hybridization buffer (HB), which contains 2x SSPE (Life Technologies, Cat15591-043), 0.05% Tween-20 (1610781, Bio-Rad), 0.05% RNase Inhibitor (2313A, Takara). .. Oligo dT25 magnetic beads (61005, Invitrogen) were prepared with three washes of 1x hybridization buffer (HB), which contains 2x SSPE (Life Technologies, Cat#15591-043), 0.05% Tween-20 (1610781, Bio-Rad), 0.05% RNase Inhibitor (2313A, Takara).

Article Title: Shared loci but distinct variants underlie genetic architecture of allergic diseases
Article Snippet: Sorted macrophages were mixed 1:1 with 2% formaldehyde (Pierce 28906) in PBS at RT with gentle rotation for 15min. .. Following cell lysis, restriction digestion, proximity ligation, and DNA sheering and size selection, purified Hi-C libraries were with 2.5μ Cot-1 (Invitrogen 15279-001), 2.5μL Salmon Sperm (Invitrogen 15632-001) and 0.5μL each of p5 and p7 blocking primers (IDT 1016184 & 1016186) in hybridization buffer (25μL 20X SSPE (Invitrogen AM9767), 1μL 0.5M EDTA (Invitrogen AM9260), 10μL 50X Denhardt’s Solution (Invitrogen 750018) and 13μL 1% SDS (Invitrogen 15553-035)). .. Following cell lysis, restriction digestion, proximity ligation, and DNA sheering and size selection, purified Hi-C libraries were with 2.5μ Cot-1 (Invitrogen 15279-001), 2.5μL Salmon Sperm (Invitrogen 15632-001) and 0.5μL each of p5 and p7 blocking primers (IDT 1016184 & 1016186) in hybridization buffer (25μL 20X SSPE (Invitrogen AM9767), 1μL 0.5M EDTA (Invitrogen AM9260), 10μL 50X Denhardt’s Solution (Invitrogen 750018) and 13μL 1% SDS (Invitrogen 15553-035)).

Membrane:

Article Title: Stochastic nature and physiological implications of 5'-NAD RNA cap in bacteria.
Article Snippet: The RNA probe was purified using Micro Bio-SpinTM P -6 Gel Columns (Biorad), denatured 5 min in 95 ◦C and cooled on ice. .. The membrane with immobilized RNA was prehybridized in buffer (1% SDS, 6X SSPE (Invitrogen), 10 × Denhardt’s Solution (Invitrogen),1M NaCl, sodium phosphate buffer pH 7.4, 50% formamide, yeast tRNA 200 mg / ml (Sigma Aldrich)) for 1h in 42 ◦C and hybridized with the RNA probe overnight at 42 ◦C. .. Detection of low abundant RNAs by northern blotting with RNA probe Less abundant sRNAs / mRNAs were detected using radiolabelled RNA probes prepared by T7 in vitro transcription with α32 P UTP (see the section ‘ In vitro transcription with T7 RNAP’).

Magnetic Beads:

other:

Article Title: Stochastic nature and physiological implications of 5′-NAD RNA cap in bacteria
Article Snippet: The RNA probe was purified using Micro Bio-Spin P -6 Gel Columns (Biorad), denatured 5 min in 95°C and cooled on ice.

Article Title: Bacteria sense the antibiotic rifampicin through a widespread dual-promoter based alarm system
Article Snippet: RNA from both types of gels was transferred onto a BrightStar-Plus Positively Charged Nylon Membrane (Invitrogen) using a semidry transfer apparatus at 15 V for 1 h (Bio-Rad).

Lysis:

Ligation:

Size Selection:

Purification:

Hi-C:

Blocking Assay:

Microarray:

Article Title: Analyzing Dehalochip: A Functional DNA Microarray for Reductive Dichlorination in Chloroethene-Contaminated Sites.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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